Detection of Borna disease virus RNA in formalin-fixed, paraffin-embedded brain tissues by nested PCR.
نویسندگان
چکیده
A method for detecting Borna disease virus (BDV) RNA in formalin-fixed, paraffin-embedded brain tissue sections was established. By digestion with proteinase K and subsequent extraction with guanidinium thiocyanate, phenol, and chloroform, we were able to efficiently release RNA from the fixed tissues. By reverse transcription of the RNA and nested PCR a 212-bp product was generated, as expected.
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عنوان ژورنال:
- Journal of clinical microbiology
دوره 33 4 شماره
صفحات -
تاریخ انتشار 1995